Anti-CLCN2 Rabbit Polyclonal Antibody for WB and ELISA - P51788

Core Product Specifications and Parameters

Biological Background: CLCN2 Function and Localization

• CLCN2 encodes the chloride channel protein 2, which forms a homodimeric channel with two independent ion conduction pathways, displaying double-barreled currents controlled by fast glutamate gates on each subunit and a slow common gate. Related references: PMID:16155254 PMID:17567819
• Inward rectification currents are activated upon membrane hyperpolarization and extracellular hypotonicity, contributing to chloride conductance that regulates neuron excitability and provides an efflux pathway to prevent dendritic chloride accumulation during GABA receptor activation. Related references: PMID:19191339
• In glial cells, association with HEPACAM/GlialCAM at astrocytic processes and myelinated fiber tracts mediates transcellular chloride flux, buffering extracellular chloride and potassium concentrations. Related references: PMID:22405205 PMID:23707145
• Channel opening at hyperpolarized potentials in adrenal glomerulosa cells causes membrane depolarization, activation of voltage-gated calcium channels, and upregulation of aldosterone synthase, thereby regulating aldosterone biosynthesis. Related references: PMID:29403011 PMID:29403012
• CLCN2 is essential for phagocytosis of shed photoreceptor outer segments in retinal pigment epithelium, facilitating photoreceptor renewal. Related references: PMID:36964785
• The protein is localized to the basolateral membrane of epithelial cells, dendritic spine membranes, and axons, underscoring its role in chloride reabsorption and neuronal signaling.
• Ubiquitously expressed, with notable presence in adrenal gland zona glomerulosa, white matter perivascular astrocytes, and ependymal cells; post-translational modifications include acetylation and phosphorylation, and mutations are linked to epilepsy.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 550–770) of human CLCN2; consider using this antibody to detect full-length protein and potentially some splice variants.
• For Western blot, a band around 99 kDa is expected; validate using lysates from human or mouse brain, adrenal gland, or transfected cell lines expressing CLCN2.
• In ELISA, ensure antigen coating conditions are optimized for the recombinant fragment used as immunogen; cross-reactivity with mouse suggests utility in rodent model studies.
• Include appropriate positive and negative controls (e.g., CLCN2 knockout or overexpression lysates) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, empowering diagnostic manufacturers and research laboratories with high-quality antibodies and ancillary reagents. This anti-CLCN2 polyclonal antibody supports robust detection in Western blot and ELISA, aiding investigations into chloride channel biology, neurological disorders, and adrenal pathophysiology. Leverage our extensive supply network for validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing—bridging the gap from concept to clinic.