Anti-CLCN1 Rabbit Monoclonal Antibody for WB and ELISA - P35523

Core Product Specifications and Parameters

Biological Background: Chloride Channel Protein 1 (CLCN1) Function and Localization

• Chloride channel protein 1 (CLCN1), also known as the skeletal muscle chloride channel, is a voltage-gated ion channel encoded by the CLCN1 gene. It belongs to the CLC family of chloride channels and transporters.
• CLCN1 is essential for skeletal muscle excitability, generating the majority of the resting chloride conductance. It stabilizes the resting membrane potential and contributes to action potential repolarization. Related references: PMID:12456816 PMID:16027167 PMID:22521272
• The channel functions as a homodimer, with each subunit containing an independent ion conduction pathway. Channel gating involves fast glutamate gates per subunit and a slow common gate that controls both pores simultaneously. Related references: PMID:10051520 PMID:10962018 PMID:29809153
• CLCN1 displays anion selectivity with a permeability sequence of chloride > thiocyanate > bromide > nitrate > iodide. Related references: PMID:9122265 PMID:9565403
• Predominantly expressed in skeletal muscle, CLCN1 localizes to the sarcolemma and T-tubule membranes.
• Post-translational phosphorylation (indicated by the phosphoprotein keyword) likely modulates channel activity.
• The protein contains two CBS domains that act as regulatory modules, and mutations in CLCN1 are associated with hereditary skeletal muscle channelopathies.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (amino acids 889-988), suggesting the antibody recognizes an intracellular epitope; verify in your specific assay format.
• For Western blotting, the predicted molecular weight is approximately 109 kDa. Use human skeletal muscle lysate as a positive control, and consider running samples under denaturing and reducing conditions.
• In ELISA, the immunizing peptide can serve as a positive control to confirm antibody binding.
• Due to the restricted tissue expression, ensure your sample system is appropriate—this target is predominantly skeletal muscle-specific.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-CLCN1 monoclonal antibody supports skeletal muscle research and diagnostics, and we can assist with sourcing rare target raw materials for your assay development pipeline. Our one-stop service covers every stage from concept to clinic.