Anti-CLCN1 Polyclonal Antibody for WB, IHC-P, ELISA - P35523

Core Product Specifications and Parameters

Biological Background: CLCN1 Function and Localization

• CLCN1 (Chloride Channel Protein 1) is a voltage-gated chloride channel essential for skeletal muscle excitability. It generates most of the plasma membrane chloride conductance, stabilizes the resting membrane potential, and contributes to action potential repolarization.
  Related references: PMID:12456816, PMID:16027167, PMID:22521272
• The channel functions as a homodimer with each subunit providing an independent ion conduction pathway. It exhibits double-barreled currents controlled by fast glutamate gates (independent per subunit) and a slow common gate, with high open probability at resting potential that increases upon depolarization.
  Related references: PMID:10051520, PMID:10962018, PMID:29809153
• CLCN1 displays anion permeability with a selectivity sequence of chloride > thiocyanate > bromide > nitrate > iodide.
  Related references: PMID:9122265, PMID:9565403
• Subcellular localization: CLCN1 is located at the cell membrane, specifically the sarcolemma and T-tubules of skeletal muscle cells.
• Tissue specificity: Predominantly expressed in skeletal muscles.
• As a phosphoprotein containing CBS domains, CLCN1 is subject to regulatory phosphorylation. UniProt keywords include 'Disease variant', indicating involvement in hereditary skeletal muscle channelopathies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 779–988) of human CLCN1, targeting a sequence that may recognize both full-length and truncated protein forms. For Western blot, consider using skeletal muscle tissue lysates or CLCN1-expressing cell lines as positive controls, and validate in your specific sample system.
• For IHC-P, membrane protein retrieval and detection may require optimized antigen retrieval protocols; consider testing citrate or EDTA-based methods.
• Reported reactivity includes human, mouse, and rat; cross-reactivity with other species should be empirically determined.
• This polyclonal antibody is suitable for multiple applications (WB, IHC-P, ELISA) but optimal dilutions and conditions must be established by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD developers, offering a wide range of raw materials from concept to clinic. For skeletal muscle and ion channel research, we supply validated antibodies, optimized polyclonal reagents like this anti-CLCN1 antibody, and bulk ancillary materials. Our procurement capabilities help you secure rare-target raw materials efficiently, supporting your diagnostic and research advancements.