Anti-CLCC1 Polyclonal Antibody for WB, IHC-P, ELISA - Q96S66

Core Product Specifications and Parameters

Biological Background: CLCC1 Function and Localization

• CLCC1, also known as MCLC, RP32, ER anion channel 1 (ERAC1), and Mid-1-related chloride channel protein, is a chloride channel CLIC-like protein encoded by the CLCC1 gene.
• It functions as an anion-selective channel with Ca(2+)-dependent and voltage-independent gating, permeable to small monovalent anions with a selectivity of bromide > chloride > nitrate > fluoride.
• Localized to the endoplasmic reticulum (ER) membrane, it mediates chloride efflux to compensate for the loss of positive charges from the ER lumen upon Ca(2+) release.
• Contributes to the maintenance of ER Ca(2+) pools and activation of the unfolded protein response, preventing accumulation of misfolded proteins, which is critical for motor neuron and retinal photoreceptor survival. Related references: PMID:25698737 PMID:30157172 PMID:37142673
• In the retina, CLCC1 is extensively expressed in the lamina cribrosa, optic nerve, ganglion cell layer, inner nuclear layer, outer nuclear layer, and retinal pigment epithelium.
• Post-translational modifications include phosphorylation, and the protein undergoes alternative splicing. It is classified under keywords such as chloride channel, ion transport, endoplasmic reticulum, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 302-501 of human CLCC1, targeting the C-terminal region; consider this when validating binding to potential isoforms or splice variants.
• Recommended applications include Western blot (WB), immunohistochemistry (paraffin-embedded, IHC-P), and ELISA. Optimize antibody dilution and experimental conditions using appropriate positive and negative controls for your sample type.
• This antibody cross-reacts with both human and mouse CLCC1, making it suitable for studies in mouse models; however, perform validation in the specific tissue or cell line of interest.
• For WB, the predicted molecular weight of CLCC1 is 62 kDa; confirm band specificity with blocking peptides or siRNA knockdown as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to a comprehensive portfolio of IVD raw materials. This CLCC1 polyclonal antibody supports research into endoplasmic reticulum stress, calcium signaling, and retinal biology, areas critical for understanding neurodegenerative and ocular diseases. Beyond single antibodies, CamelBio can source validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target raw materials to accelerate your project from concept to clinic.