Anti-CLCA1 Polyclonal Antibody for WB, IF-P, ELISA - A8K7I4

Core Product Specifications and Parameters

Biological Background: CLCA1 Function and Localization

• CLCA1, also known as calcium-activated chloride channel regulator 1 or CaCC-1, belongs to the calcium-activated chloride channel family.
• It is proposed to mediate calcium-activated chloride conductance and plays critical roles in goblet cell metaplasia, mucus hypersecretion, and airway hyperresponsiveness (AHR).
• Involved in the regulation of mucus production and secretion by goblet cells, and induces MUC5AC expression.
• Plays a role in tissue inflammation as part of the innate immune response and may function as a tumor suppressor.
• Highly expressed in the small intestine, colon (especially intestinal basal crypt epithelia and goblet cells), appendix, and bronchial epithelium; weakly expressed in uterus, testis, and kidney.
• Subcellularly localized to the cell membrane and secreted into the extracellular space.
• Features autocatalytic cleavage, glycosylation, and metal-binding domains (zinc), characteristic of metalloproteases.
• The protein has a molecular weight of approximately 100 kDa (100226 Da).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 22–150 in the N-terminal region; consider that this antibody may detect full-length and cleaved fragments.
• For Western blotting, CLCA1 migrates at ~100 kDa; validate band specificity using relevant tissue lysates (e.g., colon, small intestine).
• In IF-P, anticipate membrane and possibly secreted localization; include appropriate controls to confirm staining.
• This polyclonal antibody may cross-react with mouse and rat CLCA1; always verify in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to IVD raw materials, bridging concept-to-clinic needs for diagnostic manufacturers and research institutes. For mucus hypersecretion, inflammation, or ion transport research, we supply validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents tailored to your assay development. Contact us for bulk orders or custom sourcing of rare target reagents.