ABflo 647 Anti-Claudin18.2 Monoclonal Antibody for Flow Cytometry - P56856-2

Core Product Specifications and Parameters

Biological Background: Claudin18.2 Function and Localization

• Claudin18.2 is an isoform of Claudin-18 (isoform A2), encoded by the CLDN18 gene, and functions as an integral membrane protein at tight junctions.
• It plays a critical role in forming the gastric paracellular barrier, regulating ion and solute permeability and contributing to the response to gastric acidification.
• Claudin-18 is a member of the claudin family of tight junction proteins, which are essential for maintaining cell polarity and barrier integrity.
• Subcellularly, Claudin18.2 localizes to tight junctions at the lateral cell membrane, where it interacts with other tight junction components.
• Expression of Claudin18.2 is highly tissue-specific, restricted to the stomach mucosa; it is predominantly observed in epithelial cells of the pit region and the base of gastric glands, including both exocrine and endocrine cells (at protein level).
• The protein has a calculated molecular weight of approximately 27.7 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 1-261) of human Claudin18.2, making this antibody suitable for detecting cell surface expression in live cells without permeabilization. Consider optimizing antibody concentration for flow cytometry to minimize background.
• Since Claudin18.2 expression is restricted to stomach tissue, use appropriate positive and negative controls (e.g., gastric cell lines or primary cells) to validate specificity.
• The ABflo 647 conjugate allows direct detection, eliminating the need for secondary antibodies; ensure appropriate compensation settings in multicolor flow cytometry experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material sourcing for gastric barrier and oncology research, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Whether you need this ABflo 647-conjugated anti-Claudin18.2 antibody for flow cytometry or custom Claudin-targeting reagents, our expert team ensures seamless procurement from concept to clinic.