Anti-Claudin 6 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P56747

Core Product Specifications and Parameters

Biological Background: Claudin-6 Function and Localization

• Claudin-6, also known as Skullin, is a member of the claudin family of tight junction integral membrane proteins.
• It plays a major role in tight junction-specific obliteration of the intercellular space, contributing to paracellular barrier function.
• Beyond its structural role, claudin-6 acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells, highlighting its involvement in host-pathogen interactions.
• Subcellularly, it localizes to the cell membrane and is specifically enriched at tight junctions.
• Tissue expression profiling reveals presence in the liver, peripheral blood mononuclear cells, and hepatocarcinoma cell lines, suggesting a role in hepatic physiology and pathology.
• Post-translational modifications include phosphorylation, as indicated by proteomics identification keywords.

Experimental Guidance and Technical Tips

• The synthetic peptide immunogen (aa 119-219) targets a region that may be conserved across species, potentially explaining the antibody's cross-reactivity with mouse and rat.
• For Western blotting, a band around 23 kDa is expected; use appropriate controls like liver or hepatocarcinoma cell lysates.
• In immunohistochemistry, paraffin-embedded sections (IHC-P) of liver tissue are recommended; antigen retrieval optimization may be needed.
• For ELISA applications, the antibody can be used as a capture or detection reagent; validate in the relevant sample system.
• Since claudin-6 is expressed in PBMCs, consider using peripheral blood samples for functional assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for high-quality IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-Claudin 6 clone, and bulk ancillary reagents. Whether you are developing assays for tight junction research or hepatitis C virus entry studies, our supply chain ensures rapid access to hard-to-find targets and consistent lot-to-lot performance. Complement your workflow with our technical services covering every development stage.