Anti-Claudin 6 Polyclonal Antibody for WB and ELISA - P56747

Core Product Specifications and Parameters

Biological Background: Claudin 6 Function and Localization

• Claudin-6 (CLDN6) is a member of the claudin family of tight junction proteins, characterized by four transmembrane domains and a critical role in maintaining epithelial and endothelial barrier integrity.
• Functions as a key component in tight junction-specific obliteration of the intercellular space, regulating paracellular permeability and cell polarity.
• Acts as a host cell receptor for hepatitis C virus (HCV) entry into hepatic cells, facilitating viral infection (microbial infection).
• Subcellular localization: Primarily located at tight junctions of the cell membrane; also present in the cell membrane fraction.
• Tissue specificity: Expressed predominantly in the liver, peripheral blood mononuclear cells, and hepatocarcinoma cell lines; supports its role in liver physiology and pathology.
• Post-translational modifications include phosphorylation (phosphoprotein), which may regulate its junctional assembly and function.
• Belongs to the proteomics identification dataset and is annotated as a reference proteome component, highlighting its well-characterized expression.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from amino acids 121-220 of human Claudin 6, which corresponds to an intracellular loop region; this region is conserved across human, mouse, and rat, supporting observed cross-reactivity.
• For Western blot (WB), the predicted molecular weight is approximately 23 kDa; expect a band between 20-25 kDa under standard reducing conditions. Consider using lysates from liver tissue or hepatoma cell lines (e.g., HepG2) as positive controls.
• For ELISA, the antibody can be employed in indirect or sandwich formats; validation against recombinant Claudin 6 protein or peptide is recommended. Titration experiments may be necessary for optimal signal-to-noise ratio.
• Since the antibody is polyclonal, lot-to-lot variability could occur; include appropriate negative controls (e.g., knockdown or knockout samples) to confirm specificity.
• Cross-reactivity with mouse and rat allows for translational studies using rodent liver models; however, expression levels may differ, so pilot experiments are advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering a wide range of antibodies, proteins, and ancillary reagents tailored to tight junction and viral entry research. Our polyclonal anti-Claudin 6 antibody supports HCV and liver biology assay development, backed by reliable supply chains and technical consultation. From validated antibody pairs to bulk recombinant proteins, CamelBio streamlines procurement for diagnostic manufacturers and research laboratories aiming to advance from concept to clinic.