Anti-Claudin 5 Monoclonal Antibody for WB, IF/ICC, IHC-P, IP, ELISA - O00501

Core Product Specifications and Parameters

Biological Background: Claudin 5 Function and Localization

• Claudin-5, encoded by the CLDN5 gene, is also known as TMDVCF (Transmembrane protein deleted in VCFS).
• It belongs to the claudin family of integral membrane proteins that are critical for the formation of tight junctions and the regulation of paracellular permeability.
• Claudin-5 plays a major role in the obliteration of the intercellular space at tight junctions, contributing to the barrier function of epithelia and endothelia.
• The protein localizes to cell junctions, specifically tight junctions, and the cell membrane, where it associates with other junctional components.
• Claudin-5 is a multi-pass transmembrane protein with four transmembrane helices, and its molecular weight is approximately 23 kDa.

Experimental Guidance and Technical Tips

• The immunogen was designed based on a synthetic peptide within human Claudin-5; consider validating specificity by peptide blocking assay or knockout/knockdown experiments in relevant cell lines.
• For western blotting, a band at ~23 kDa is expected; optimize antibody dilution and use appropriate positive controls (e.g., endothelial or epithelial cell lysates).
• In immunofluorescence and immunohistochemistry, claudin-5 typically shows membrane and tight junction staining; use proper fixation and permeabilization conditions to maintain epitope accessibility.
• This antibody is also suitable for immunoprecipitation and ELISA; pilot experiments are recommended to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a streamlined path from concept to clinic, supplying validated anti-Claudin 5 antibodies, matched antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Whether developing tight junction biomarker detection kits or exploring cell junction biology, CamelBio bridges sourcing gaps with reliable raw materials and flexible support tailored to your IVD development needs.