Anti-Claudin-3 Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - O15551

Core Product Specifications and Parameters

Biological Background: Claudin-3 Function and Localization

• Claudin-3, also known as CPE-receptor 2 or hRVP1, is an integral membrane protein that forms tight junction strands, essential for paracellular barrier integrity.
• It functions as a barrier-forming claudin, mediating calcium-independent cell adhesion to obliterate intercellular space at tight junctions.
• Claudin-3 is localized at cell-cell junctions, specifically within tight junctions, and is anchored to the cell membrane via multiple transmembrane helices.
• The protein undergoes phosphorylation (a key post-translational modification) that likely regulates its assembly and function in tight junction dynamics.
• Claudin-3 is linked to Williams-Beuren syndrome in curated databases, suggesting a role in developmental or neurological processes associated with this disorder.
• With a molecular weight of approximately 23 kDa (UniProt: 23319 Da), it is a relatively small claudin member, facilitating its integration into tight junction complexes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 141-220), which may allow detection of both full-length and potential truncated isoforms in lysates—verify band patterns in relevant cell lines.
• For Western blot, consider using low-percentage gels (e.g., 12%) given the 23 kDa size; include membrane and cytosolic fraction controls to confirm tight junction specificity.
• In immunofluorescence (IF/ICC), paraformaldehyde fixation and Triton X-100 permeabilization are suitable; co-stain with a tight junction marker (e.g., ZO-1) to confirm localization.
• Optimize antibody dilutions for each application using a known positive sample (e.g., human epithelial cells) to minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a full range of IVD raw materials, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents, supporting your work on tight junction biology and cell-cell adhesion. Our expertise in rare-target sourcing ensures timely access to critical reagents like this anti-Claudin-3 antibody, streamlining your development from concept to clinic.