Anti-Claudin-3 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O15551

Core Product Specifications and Parameters

Biological Background: Claudin-3 Function and Localization

• Claudin-3 functions as a barrier-forming claudin, playing a major role in tight junction-specific obliteration of the intercellular space through calcium-independent cell-adhesion activity.
• It is a transmembrane protein prominently localized at tight junctions and the cell membrane.
• Also known as Clostridium perfringens enterotoxin receptor 2 (CPE-R2) and rat ventral prostate.1 protein homolog (hRVP1).
• It belongs to the claudin family, which are essential components of tight junction strands regulating paracellular transport.
• Annotated as a phosphoprotein, indicating potential regulation by phosphorylation events.
• The protein has a calculated molecular weight of approximately 23 kDa (23319 Da) and is encoded by the CLDN3 gene located on chromosome 7.
• Keywords include: Cell junction, Membrane, Tight junction, Transmembrane helix, and Williams-Beuren syndrome region association.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide spanning amino acids 121–220 of human Claudin-3 (NP_001297.1); consider the targeted domain's topology when designing detection strategies.
• For western blotting, anticipate a band around 23 kDa under reducing conditions; optimize protein loading and transfer for low molecular weight proteins.
• In immunohistochemistry and immunofluorescence, include appropriate antigen retrieval methods and permeabilization steps to enhance epitope accessibility.
• Validate antibody reactivity with positive and negative controls using human cell lines known to express tight junction proteins (e.g., epithelial cells).
• For ELISA applications, confirm coating antigen identity and antibody specificity through indirect or sandwich ELISA optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. Our offerings include validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. With expertise in tight junction and cell membrane research, we support rare target sourcing and supply chain needs to accelerate your projects from concept to clinic.