Anti-Claudin 1 Rabbit Monoclonal Antibody for WB, IP, IF-P, IHC-P, ELISA - O95832

Core Product Specifications and Parameters

Biological Background: Claudin-1 Function and Localization

• Functions as a major constituent of tight junction complexes, regulating epithelial permeability by forming selective barriers together with other claudin family members.
• Required for normal epidermal barrier function and water homeostasis, preventing paracellular diffusion of small molecules and excessive water loss through the skin, likely via indirect effects on other protein expression.

Related references: PMID:23407391

• Acts as a co-receptor for hepatitis C virus (HCV) in hepatocytes by associating with CD81, forming an essential entry complex.

Related references: PMID:17325668 PMID:20375010 PMID:24038151

• Serves as a receptor for dengue virus entry into host cells.

Related references: PMID:24074594

• Localizes to cell-cell tight junctions, the cell membrane, and specifically the basolateral cell membrane.
• Strongly expressed in liver and kidney, with lower expression in heart, brain, spleen, lung, and testis.
• Contains conserved disulfide bonds; mutations in CLDN1 are associated with ichthyosis.
• Also known as senescence-associated epithelial membrane protein (SEMP1), highlighting roles in cell aging.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide; consider peptide blocking experiments to confirm signal specificity.
• For Western blot, the predicted molecular weight is approximately 23 kDa. Validate migration in relevant tissue/cell lysates.
• Optimize antigen retrieval for IHC-P and IF-P applications to expose epitopes in formalin-fixed, paraffin-embedded samples.
• As a transmembrane protein, use mild detergents or membrane extraction kits for optimal solubilization in IP experiments.
• For ELISA, verify coating conditions and antibody pair compatibility if developing a sandwich assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, covering all stages from concept to clinic. This anti-Claudin 1 antibody supports epithelial barrier and viral entry research, and we can also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare targets to streamline your assay development.