Anti-Claudin 1 Polyclonal Antibody for WB & ELISA - O95832

Core Product Specifications and Parameters

Biological Background: Claudin-1 Function and Localization

• Claudin-1 is a member of the claudin family of integral membrane proteins, which are major constituents of tight junctions and regulate paracellular permeability.
• It is essential for the epidermal barrier, preventing the paracellular diffusion of small molecules and maintaining skin water homeostasis. Related references: PMID:23407391
• Claudin-1 acts as a co-receptor for hepatitis C virus (HCV) entry into hepatocytes, associating with CD81 to form a receptor complex. It also functions as a receptor for dengue virus. Related references: PMID:17325668 PMID:20375010 PMID:24074594
• Subcellularly, claudin-1 localizes to tight junctions and the basolateral cell membrane.
• It is strongly expressed in liver and kidney, and also found in heart, brain, spleen, lung, and testis.
• Keywords associated with claudin-1 include cell junction, tight junction, transmembrane, disulfide bond, host cell receptor for virus entry, and ichthyosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 137-211 of human Claudin-1. For western blotting, consider using reducing conditions; the expected molecular weight is ~23 kDa.
• For ELISA applications, this antibody may be suitable for direct or indirect detection of Claudin-1 peptide or protein. Validate specificity in relevant sample systems such as liver or kidney tissue lysates.
• As a polyclonal antibody, lot-to-lot consistency should be verified, and optimal working dilutions must be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-Claudin 1 antibody, and bulk ancillary reagents. Our expert team supports rare target sourcing and tailored supply solutions for tight junction research, viral entry studies, and skin barrier disorder investigations. From discovery to diagnostics, CamelBio bridges your sourcing needs.