Anti-Clathrin heavy chain Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q00610

Core Product Specifications and Parameters

Biological Background: Clathrin heavy chain 1 Function and Localization

• Clathrin heavy chain 1 (CLTC, also known as CLH-17) is the major structural component of the polyhedral coat of coated pits and vesicles, essential for receptor-mediated endocytosis and intracellular trafficking.
• Acts as a component of the TACC3/ch-TOG/clathrin complex, contributing to kinetochore fiber stabilization by forming inter-microtubule bridges, and is required for maintenance of kinetochore fiber tension (PubMed:15858577, PubMed:16968737, PubMed:21297582, PubMed:23532825). Related references: PMID:15858577 PMID:16968737 PMID:21297582 PMID:23532825
• Involved in early autophagosome formation (PubMed:20639872). Related references: PMID:20639872
• Interaction with DNAJC6 mediates the recruitment of HSPA8 to the clathrin lattice, creating local destabilization that promotes uncoating (By similarity).
• Subcellular localization includes cytoplasmic vesicle membranes, coated pits, melanosomes, and the mitotic spindle, reflecting its diverse roles in membrane traffic and cell division.
• Post-translational modifications include acetylation and phosphorylation, and it is implicated in processes such as cell cycle progression, mitosis, and autophagy.
• Alternative splicing generates multiple isoforms; the canonical isoform has a molecular weight of approximately 192 kDa (191,615 Da per UniProt).

Experimental Guidance and Technical Tips

• The immunogen (aa 1451-1675) resides within a conserved C-terminal region, supporting applications such as western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA; optimizing dilution factors for each application is recommended.
• Due to high sequence identity across species, cross-reactivity with mouse and rat is expected; if using non-human samples, validate in the relevant sample system.
• For WB, note the large molecular weight (~192 kDa); use appropriate gel percentages (e.g., 6–8% SDS-PAGE) and extended transfer times for efficient blotting.
• As a rabbit polyclonal antibody, it may recognize multiple epitopes, enhancing signal but requiring careful specificity controls such as knockout validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, providing access to validated antibodies, optimized antibody pairs, and bulk ancillary reagents for targets such as Clathrin heavy chain, pivotal in endocytosis and cell cycle research. From polyclonal antibodies to rare-target raw materials, we support diagnostic manufacturers and labs from concept to clinic with reliable supply solutions.