Anti-Clathrin Heavy Chain Rabbit Monoclonal Antibody for WB, IHC, IF, IP, ELISA - Q00610 / P53675

Core Product Specifications and Parameters

Biological Background: Clathrin Heavy Chain Function and Localization

• Clathrin heavy chain 1 (CLTC) is the major structural component of the polyhedral coat of clathrin-coated pits and vesicles, mediating both plasma membrane and trans-Golgi network trafficking.
• Acts as a component of the TACC3/ch-TOG/clathrin complex that stabilizes kinetochore fibers of the mitotic spindle by bridging microtubules, essential for maintaining kinetochore fiber tension during mitosis. Related references: PMID:15858577 PMID:16968737 PMID:21297582 PMID:23532825
• Plays a role in early autophagosome formation. Related references: PMID:20639872
• Interaction with DNAJC6 recruits HSPA8 to the clathrin lattice, locally destabilizing it to promote uncoating (By similarity).
• Localizes to cytoplasmic vesicle membranes, coated pits, melanosomes, and the mitotic spindle, reflecting its diverse cellular functions.
• Subject to post-translational modifications including acetylation and phosphorylation, with alternative splicing generating multiple isoforms.
• The gene CLTC (chromosome 17) encodes this 191.6 kDa protein; a closely related heavy chain (CLTCL1, encoded by a separate gene) shares high homology and is also recognized by this antibody (as indicated by cross-reactivity to P53675).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide within the N-terminal region (aa 200–300), which is conserved across human, mouse, and rat, consistent with the observed cross-reactivity. Validate dilution and detection conditions for each application in your specific sample system.
• For Western blotting, the predicted molecular weight of ~191 kDa may require extended transfer times or low-percentage gels. Consider using a high-molecular-weight protein standard.
• In immunoprecipitation, the antibody's rabbit monoclonal nature may provide high specificity; however, clathrin's large size and complex formation may necessitate optimization of lysis buffers.
• For IF/ICC and IHC-P, fixation and permeabilization conditions should be optimized to preserve clathrin-coated structures.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined procurement channel for high-quality IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. This anti-Clathrin heavy chain monoclonal antibody exemplifies our commitment to supporting cellular trafficking and mitosis research with reliable tools. For customized bulk orders or assistance in sourcing rare targets, our team provides end-to-end technical and supply chain support.