Anti-Clathrin heavy chain Polyclonal Antibody for WB and ELISA - Q00610

Core Product Specifications and Parameters

Biological Background: Clathrin heavy chain 1 Function and Localization

• Clathrin heavy chain 1 (CLTC) is a major structural protein of coated pits and vesicles, also known as clathrin heavy chain on chromosome 17 (CLH-17).
• Clathrin forms the polyhedral coat of coated pits and vesicles, mediating endocytosis and intracellular trafficking through interactions with adapter protein complexes at the plasma membrane and trans-Golgi network.
• Acts as a component of the TACC3/ch-TOG/clathrin complex, which contributes to stabilization of kinetochore fibers of the mitotic spindle by acting as an inter-microtubule bridge and is required for maintenance of kinetochore fiber tension. Related references: PMID:15858577 PMID:16968737 PMID:21297582 PMID:23532825
• Plays a role in early autophagosome formation. Related reference: PMID:20639872
• Interaction with DNAJC6 is thought to mediate recruitment of HSPA8 to the clathrin lattice, creating local destabilization that promotes uncoating of clathrin cages.
• Subcellularly localized to cytoplasmic vesicle membranes, coated pits, melanosomes, the cytoplasm, cytoskeleton, and mitotic spindle.
• Subject to acetylation and phosphorylation, with alternative splicing producing multiple isoforms; mutations are associated with intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide from aa 1550-1650 of human Clathrin heavy chain; consider that this region is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot (WB), a band around 192kDa is expected; consider optimizing lysate preparation and gel conditions for large proteins.
• For ELISA, validate the antibody with the relevant recombinant protein or peptide to confirm specificity.
• As a polyclonal antibody, it may recognize multiple clathrin isoforms; confirm specificity in your system using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for IVD raw materials, including validated antibody pairs, optimized mono- and polyclonal antibodies, and bulk ancillary reagents for diagnostic development. This anti-Clathrin heavy chain antibody supports research in endocytosis, intracellular trafficking, mitosis, and autophagy. For rare target raw-material sourcing or custom development needs, CamelBio bridges the gap from concept to clinic.