Anti-Clathrin Heavy Chain Monoclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - Q00610

Core Product Specifications and Parameters

Biological Background: Clathrin Heavy Chain 1 Function and Localization

• Clathrin heavy chain 1 (CLTC) constitutes the major structural component of the polyhedral lattice that coats endocytic pits and transport vesicles, playing a critical role in receptor-mediated endocytosis and intracellular membrane trafficking.
• Two distinct adaptor protein complexes (AP-2 and AP-1) link the clathrin lattice to the plasma membrane and the trans-Golgi network, respectively, sorting cargo into emerging vesicles.
• CLTC forms a complex with TACC3 and ch-TOG that stabilizes kinetochore fibers during mitosis by bridging microtubules, contributing to spindle integrity.
  Related references: PMID:15858577, PMID:16968737, PMID:21297582
• The TACC3/ch-TOG/clathrin complex is essential for the maintenance of proper kinetochore fiber tension, ensuring accurate chromosome segregation.
  Related references: PMID:23532825
• CLTC participates in early autophagosome formation, highlighting a role in autophagy-related membrane remodeling.
  Related references: PMID:20639872
• Subcellularly, clathrin heavy chain 1 localizes to cytoplasmic vesicle membranes, coated pits, melanosomes, and the mitotic spindle, reflecting its diverse trafficking and cell-division functions.
• Key post‑translational modifications include acetylation and phosphorylation, and the protein is subject to alternative splicing, with implications for lattice dynamics and disease (e.g., intellectual disability‑associated variants).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C‑terminal peptide (aa 1571‑1670) of human CLTC; the epitope region is likely conserved across human, mouse, and rat, supporting the listed cross‑reactivity. Validate reactivity in your specific sample system.
• For western blot (WB), the observed molecular weight is approximately 187 kDa; ensure adequate transfer time and membrane handling for high‑molecular‑weight proteins.
• The rabbit monoclonal format provides high specificity and batch‑to‑batch consistency; consider using it as a reliable detection tool for clathrin in immunoprecipitation (IP) and immunofluorescence (IF/ICC).
• In immunohistochemistry (IHC‑P), antigen retrieval conditions may require optimization; a standard citrate‑based heat‑induced epitope retrieval (HIER) protocol is a common starting point.
• For ELISA applications, the purified monoclonal antibody can serve as a capture or detection reagent in dedicated assay development; pair matching tests are advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with comprehensive access to premium IVD raw materials, technical expertise, and seamless supply-chain solutions—from initial concept through clinical implementation. Whether you need validated antibody pairs, optimized monoclonal antibodies like this anti‑clathrin heavy chain reagent, or bulk ancillary materials for vesicle trafficking and endocytosis research, CamelBio streamlines your procurement. Contact us to discuss custom sourcing requests and accelerate your assay development.