Anti-CKS2 Polyclonal Antibody for WB, ELISA - P33552

Core Product Specifications and Parameters

Biological Background: CKS2 Function and Localization

• CKS2 (Cyclin-dependent kinases regulatory subunit 2) is a small protein of approximately 9.9 kDa that belongs to the CKS (cyclin-dependent kinase subunit) family, which also includes CKS1.
• It encodes a regulatory subunit that binds directly to the catalytic subunit of cyclin-dependent kinases (CDKs) and is essential for their biological function in cell cycle progression.
• CKS2 plays a key role in the control of cell division, particularly in the regulation of mitosis and the G2/M transition.
• The protein is subject to post-translational acetylation, which may influence its stability or interactions.
• Consistent with its role in the cell cycle, CKS2 is widely expressed in proliferating tissues, though detailed tissue specificity has not been characterized in this record.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a recombinant protein covering full-length human CKS2 (amino acids 1–79). This design should target the entire functional protein and recognize both native and denatured forms.
• For Western blot (WB), consider using a 15% SDS-PAGE gel to resolve the ~10 kDa protein; validate with a positive control such as HeLa or HEK293 cell lysates, where CKS2 is expected to be expressed.
• The antibody is reported to cross-react with rat CKS2; verify cross-reactivity in your rat sample system, as sequence homology between species may affect antibody affinity.
• ELISA applications should be optimized by testing a range of coating antigen concentrations and antibody dilutions; include a blocking step with BSA to reduce background.
• As this is a polyclonal antibody, lot-to-lot variability may occur. We recommend including appropriate positive and negative controls in every experiment to confirm consistent reactivity.

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