Anti-CKMT2 (SMTCK) Polyclonal Antibody for WB and ELISA - P17540

Core Product Specifications and Parameters

Biological Background: CKMT2 Function and Localization

• CKMT2 encodes the sarcomeric mitochondrial creatine kinase (S-MtCK), also known as basic-type mitochondrial creatine kinase (Mib-CK), a key enzyme in cellular energy homeostasis.
• Reversibly catalyzes the transfer of phosphate between ATP and creatine phosphate, playing a central role in energy transduction in tissues with high and fluctuating energy demands, such as skeletal muscle, heart, brain, and spermatozoa.
• Part of the creatine kinase family; distinct from cytosolic isoforms, CKMT2 localizes to the mitochondrial intermembrane space and is specifically expressed in sarcomeric tissues.
• Located at the mitochondrial inner membrane, coupling mitochondrial ATP production with cytosolic energy demands.
• Expression is restricted to heart and skeletal muscles (sarcomere-specific), consistent with its role in muscle energy metabolism.
• Identified as a phosphoprotein and contains ATP- and nucleotide-binding domains; features a transit peptide for mitochondrial import.
• The mature protein has a molecular weight of approximately 47.5 kDa (UniProt), closely matching the ~48 kDa band observed in assays.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 40–230 of human CKMT2, corresponding to a region within the mature protein after cleavage of the mitochondrial transit peptide (amino acids 1–39). This design likely yields an antibody that recognizes the processed form.
• For Western blotting, a band around 48 kDa is expected; consider using mitochondrial-enriched lysates from heart or skeletal muscle tissue for optimal detection.
• The antibody cross-reacts with mouse and rat CKMT2 based on sequence homology; validation in the specific experimental system is advised.
• Recommended applications are WB and ELISA; suitability for other techniques (e.g., IHC, IF) should be tested individually.
• As with all polyclonal antibodies, lot-to-lot consistency can be monitored by including appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides reliable sourcing of IVD raw materials, including this anti-CKMT2 polyclonal antibody, to support research in mitochondrial biology, energy metabolism, and muscle-related diseases. Our portfolio encompasses validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents, with expertise in supplying rare targets. Partner with us for streamlined procurement that accelerates your diagnostic development from early R&D to clinical application.