Anti-CKMT2 Rabbit Polyclonal Antibody for WB and ELISA - P17540

Core Product Specifications and Parameters

Biological Background: CKMT2 Function and Localization

• CKMT2 encodes the sarcomeric mitochondrial creatine kinase (S-MtCK), also known as basic-type mitochondrial creatine kinase (Mib-CK), a pivotal enzyme in cellular energy homeostasis.
• The enzyme reversibly catalyzes phosphate transfer between ATP and creatine phosphate, playing a central role in energy transduction in tissues with high and fluctuating energy demands, such as skeletal muscle, heart, brain, and spermatozoa.
• CKMT2 is specifically expressed in sarcomeric tissues, predominantly in heart and skeletal muscles, as indicated by its tissue specificity.
• The protein localizes to the mitochondrial inner membrane, where it facilitates the efficient coupling of ATP production to energy utilization.
• It belongs to the creatine kinase family of isoenzymes and undergoes phosphorylation, as suggested by the keyword "Phosphoprotein".
• UniProt keywords—including ATP-binding, kinase, transferase, membrane, and mitochondrion inner membrane—underscore its functional role in mitochondrial energy metabolism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 40–230 of human CKMT2, a central region that may confer epitope specificity; consider that the antibody might not recognize truncated or highly modified isoforms.
• For Western blot, expect a band near 48 kDa; validate detection using lysates from heart or skeletal muscle, where CKMT2 is abundantly expressed.
• When performing ELISA, optimize coating antigen concentration and antibody dilution using recombinant protein or tissue extracts, and include appropriate controls.
• Given reported cross-reactivity with human, mouse, and rat, the antibody can be used across these species for comparative studies, but always verify species-specific performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a seamless, one-stop solution for IVD raw material sourcing. For mitochondrial creatine kinase studies, we supply validated anti-CKMT2 polyclonal antibodies like this one, alongside custom monoclonal antibody development, bulk ancillary reagents, and rare target sourcing services. Our integrated platform supports projects from concept to clinic, ensuring reliable access to high-quality raw materials tailored to your assay needs.