Anti-CKMT1B Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P12532

Core Product Specifications and Parameters

Biological Background: CKMT1B Function and Localization

• CKMT1B encodes the ubiquitous mitochondrial creatine kinase (U-MtCK), also known as acidic-type mitochondrial creatine kinase (Mia-CK). It is part of a gene family that includes CKMT1A, and both genes undergo alternative splicing to produce isoforms.
• The enzyme reversibly catalyzes phosphate transfer between ATP and creatine phosphate, playing a central role in cellular energy homeostasis in tissues with high and fluctuating energy demands.
• CKMT1B is localized to the mitochondrial inner membrane, where it facilitates efficient energy transduction by coupling mitochondrial ATP production with cytosolic phosphocreatine pools.
• Its expression is ubiquitous, with particularly high levels in skeletal muscle, heart, brain, and spermatozoa, reflecting the energy requirements of these tissues.
• The protein is a phosphoprotein and a kinase, features consistent with its regulatory role in energy metabolism and signal transduction.
• A mitochondrial transit peptide directs the protein to the mitochondrion, and upon import, it becomes anchored to the inner membrane.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-85), which may influence epitope recognition. Consider using standard denaturing conditions for Western blot (WB) analysis.
• For immunofluorescence (IF/ICC), expect a mitochondrial staining pattern. Include appropriate organelle markers or co-stains to confirm specificity.
• In immunoprecipitation (IP) assays, use optimized lysis buffers that preserve native protein conformation. The antibody's polyclonal nature may aid in capturing different conformations or complexes.
• Since cross-reactivity is reported for human, mouse, and rat, validate the antibody in your specific sample system using appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-CKMT1B antibody supports research into mitochondrial energy metabolism, muscle physiology, and related disease areas. Our sourcing capabilities extend to rare targets and custom solutions, simplifying procurement for diagnostic manufacturers and research laboratories.