Anti-CKMT1A Rabbit Polyclonal Antibody for WB, ELISA - P12532

Core Product Specifications and Parameters

Biological Background: CKMT1A Function and Localization

• CKMT1A encodes the ubiquitous mitochondrial creatine kinase (U-MtCK), also known as acidic-type mitochondrial creatine kinase (Mia-CK). It is a member of the creatine kinase isoenzyme family with two variant genes: CKMT1A and CKMT1B.
• The enzyme reversibly catalyzes phosphate transfer between ATP and creatine phosphate, a critical reaction for energy homeostasis in high-demand tissues like skeletal muscle, heart, brain, and spermatozoa.
• Localized to the mitochondrial inner membrane, it bridges mitochondrial ATP production and cytosolic energy consumption.
• The protein harbors ATP-binding and nucleotide-binding domains, characteristic of kinases and transferases, and undergoes alternative splicing.
• Post-translational modifications include phosphorylation, and the protein contains a mitochondrial transit peptide for targeting.
• UniProt lists it under multiple keywords: 3D-structure, alternative splicing, ATP-binding, kinase, membrane, mitochondrion, mitochondrion inner membrane, nucleotide-binding, phosphoprotein, proteomics identification, reference proteome, transferase, transit peptide.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 258–417) of human CKMT1A, which may provide isoform specificity toward the U-type mitochondrial creatine kinase.
• In Western blot, the expected band is approximately 47 kDa; validate using mitochondrial-enriched lysates from heart or brain tissue.
• ELISA application could be developed for quantitative detection; consider pairing with a monoclonal detection antibody for improved specificity.
• Reported cross-reactivity with mouse and rat supports translational research but always verify in the relevant model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our CKMT1A antibody supports research on mitochondrial creatine kinase function, and we provide comprehensive sourcing solutions from discovery to clinical application, including rare target raw materials and custom technical services.