Anti-CKLF Rabbit Polyclonal Antibody for WB, ELISA - Q9UBR5

Core Product Specifications and Parameters

Biological Background: CKLF Function and Localization

• CKLF (Chemokine-like factor), also known as C32, is a member of the chemokine-like factor family and produces multiple isoforms (CKLF1-4) through alternative splicing.
• It plays an important role in inflammation and regeneration of skeletal muscle (PubMed:11415443).
  Related references:
  PMID:11415443
• CKLF has chemotactic activity on monocytes, neutrophils, and lymphocytes, directing their migration (PubMed:11415443).
  Related references:
  PMID:11415443
• The protein binds to the chemokine receptor CCR4, mediating its downstream effects (PubMed:16137713).
  Related references:
  PMID:16137713
• CKLF is essential for embryonic development, as suggested by similarity data.
• Subcellularly, CKLF is located in secreted and membrane compartments, featuring transmembrane helix motifs that support its dual localization.
• The isoforms exhibit highest expression in adult spleen, lung, testis, ovary, peripheral blood leukocyte, placenta, and pancreas, as well as in fetal brain, skeletal muscle, thymus, and heart, with lower levels in several other tissues.
• Key structural and functional keywords include alternative splicing, chemotaxis, cytokine, membrane, and secreted.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–67) of human CKLF; therefore, the antibody is expected to recognize full-length CKLF and possibly isoforms containing this domain.
• For Western blotting, a primary band near 17 kDa is anticipated; however, due to alternative splicing and potential post-translational modifications, additional bands may be observed. Include appropriate positive and negative controls.
• When using ELISA, consider using recombinant CKLF protein as a standard and optimize antibody concentration for detection.
• Cross-reactivity has been reported for mouse and rat; nevertheless, validate the antibody in your specific sample system to confirm reactivity.
• As a polyclonal antibody, it may exhibit lot-to-lot variability; include a previous validated lot as a reference if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD raw materials, supporting your project from concept to clinic. This CKLF antibody is tailored for immunology and developmental research, and alongside it we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our team also facilitates rare target material sourcing, ensuring you have a reliable partner for your diagnostic and research needs.