Anti-CKAP5 Rabbit Polyclonal Antibody for WB, IP, IF/ICC, IF-P, ELISA - Q14008

Core Product Specifications and Parameters

Biological Background: CKAP5 Function and Localization

• CKAP5 (Cytoskeleton-associated protein 5), also known as ch-TOG (Colonic and hepatic tumor overexpressed gene protein), is a large microtubule-associated protein encoded by the CKAP5 gene (GeneID: 9793) with a predicted molecular weight of ~225 kDa.
• It acts as a processive microtubule polymerase, binding to the plus ends of microtubules and promoting cytoplasmic microtubule nucleation and elongation.
• CKAP5 plays a critical role in mitotic spindle assembly by organizing spindle poles and protecting kinetochore microtubules from depolymerization.
• It is a component of the TACC3/ch-TOG/clathrin complex, which stabilizes kinetochore fibers of the mitotic spindle by acting as an inter-microtubule bridge. Related references: PMID:23532825
• CKAP5 enhances the strength of NDC80 complex-mediated kinetochore-tip microtubule attachments. Related references: PMID:27156448
• Subcellularly, CKAP5 localizes to the cytoplasm, centrosome, spindle poles, and kinetochores during cell division.
• Expression is elevated in hepatic and colonic tumors, as well as in the Purkinje cell bodies of the cerebellum, with widespread tissue distribution including skeletal muscle, brain, heart, placenta, lung, liver, kidney, and pancreas.
• Post-translational modifications include acetylation and phosphorylation, and alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1763-2032 of human CKAP5; therefore, the antibody may recognize epitopes within this C-terminal region. Consider using appropriate positive controls (e.g., HeLa, HEK293 cell lysates) for WB.
• Due to the large molecular weight (225 kDa), ensure the use of low-percentage SDS-PAGE gels and extended transfer times for Western blotting.
• For immunofluorescence applications, expect staining patterns consistent with microtubule networks, centrosomes, and mitotic spindles. Co-staining with centrosomal markers (e.g., γ-tubulin) can help confirm localization.
• Given its polyclonal nature, lot-to-lot consistency should be verified, and antibody titration is recommended for optimal signal-to-noise ratio.

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