Anti-CKAP4 Polyclonal Antibody for WB, IF/ICC, ELISA - Q07065

Core Product Specifications and Parameters

Biological Background: CKAP4 Function and Localization

• Cytoskeleton-associated protein 4 (CKAP4), also known as p63, CLIMP-63, or ERGIC-63, is a 63–66 kDa type II transmembrane protein predominantly localized to the rough endoplasmic reticulum (ER).
• CKAP4 serves as a high-affinity epithelial cell surface receptor for antiproliferative factor (APF), transducing antiproliferative signals upon ligand binding.
• It mediates the anchoring of the ER to the microtubule cytoskeleton, contributing to the maintenance of ER morphology and intracellular trafficking.
• A large cytoplasmic coiled-coil domain facilitates oligomerization and microtubule interaction, while a short luminal domain may participate in protein–protein interactions within the ER.
• Subcellular distribution includes the ER membrane, plasma membrane, perinuclear cytoplasm, and the cytoskeleton; the protein shuttles between the ER and cell surface.
• Post-translational modifications such as phosphorylation, palmitoylation, and acetylation regulate CKAP4’s function, localization, and stability.
• CKAP4 is classified as a lipoprotein with a signal-anchor transmembrane segment, underscoring its role in membrane dynamics.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 130–400, encompassing the cytoplasmic coiled-coil domain; this region is conserved across human, mouse, and rat, supporting species cross-reactivity.
• For western blot (WB), anticipate a band near 66 kDa; validate specificity using cell lines with confirmed CKAP4 expression and appropriate controls.
• In immunofluorescence (IF)/ICC, optimize permeabilization to detect both ER and plasma membrane pools; co-stain with organelle markers (e.g., calnexin for ER) to confirm localization.
• ELISA applications may benefit from using recombinant CKAP4 protein as a standard; consider coating conditions and antibody dilution for optimal signal.
• Verify antibody performance in the relevant sample system, as epitope accessibility may vary with fixation or protein context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop provider of high-quality IVD raw materials, supporting diagnostic manufacturers and research institutions from concept to clinic. This CKAP4 polyclonal antibody is part of our extensive portfolio for cancer, cytoskeleton, and membrane protein research. We also offer custom antibody development, validated monoclonal/polyclonal pairs, bulk ancillary reagents, and sourcing for rare targets to streamline your assay development.