Anti-CK14 Rabbit Polyclonal Antibody for WB, IF, ELISA - P02533

Core Product Specifications and Parameters

Biological Background: CK14 Function and Localization

• Keratin, type I cytoskeletal 14 (CK14) is an intermediate filament protein encoded by the KRT14 gene, critical for structural integrity of basal keratinocytes.
• Forms obligate heteropolymers with type II keratin KRT5, contributing to mechanical strength and resilience of the epidermal basal layer. Related references: PMID:11724817, PMID:1694855, PMID:1720261
• The nonhelical tail domain facilitates filament bundling and enhances mechanical properties of keratin networks. Related references: PMID:11724817
• Subcellular localization: Cytoplasm, Nucleus.
• Tissue specificity: Expressed in corneal epithelium, basal layer of epidermis (absent in stratum corneum), and outer root sheath of anagen hair follicles.
• Post-translational modifications and disease associations: Phosphoprotein, disulfide bonds, Ubl conjugation; related to epidermolysis bullosa, ectodermal dysplasia, and palmoplantar keratoderma.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 372-472 of human CK14; reactivity to mouse and rat suggests sequence conservation; validate in your specific sample system.
• For Western blot, the predicted band is ~51 kDa; pre-adsorption may reduce non-specific binding.
• For immunofluorescence (IF/ICC, IF-P), optimize fixation/permeabilization protocols as keratins are intermediate filaments; consider co-staining with KRT5 to confirm basal layer specificity.
• For ELISA, pair with a suitable detection antibody; the polyclonal nature may provide sensitive detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your IVD development with a comprehensive portfolio of raw materials, including validated antibody pairs, optimized recombinant antibodies, and bulk reagents for epithelial biology and dermatology research. This CK14 antibody exemplifies our commitment to providing high-quality, rare-target raw materials to accelerate your diagnostic assays from concept to clinic.