Anti-Citrate synthetase Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O75390

Core Product Specifications and Parameters

Biological Background: Citrate synthase Function and Localization

• Citrate synthase (CS) is a mitochondrial enzyme encoded by the CS gene in humans.
• It catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, the first and rate-limiting step of the tricarboxylic acid (TCA) cycle.
• CS is localized to the mitochondrial matrix, where it operates within the TCA cycle to generate energy and metabolic intermediates.
• The protein contains a mitochondrial transit peptide for import and is subject to post-translational modifications, including acetylation, methylation, and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved N-terminal region (aa 28–145) of human citrate synthase, suitable for detecting total CS protein across human, mouse, and rat samples.
• In Western blot, expect a band near 52 kDa; mitochondrial-enriched lysates may serve as a positive control.
• For IHC-P and IF/ICC, optimize antigen retrieval and permeabilization protocols to access the mitochondrial matrix.
• ELISA can be employed for quantitative analysis; validate dilution factors in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with reliable access to IVD raw materials for mitochondrial metabolism and TCA cycle research. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets—helping streamline your assay development from concept to clinic.