Anti-Citrate Synthase Monoclonal Antibody for WB - O75390

Core Product Specifications and Parameters

Biological Background: Citrate Synthase Function and Localization

• Citrate synthase (CS) is a mitochondrial enzyme encoded by the CS gene in humans, also known as citrate (Si)-synthase.
• It catalyzes the first reaction of the Krebs (TCA) cycle: the condensation of acetyl-CoA and oxaloacetate to form citrate, a critical step in cellular energy production.
• CS is localized to the mitochondrial matrix, consistent with its role in aerobic respiration.
• The enzyme is subject to post-translational modifications including acetylation, methylation, and ubiquitination, which may regulate its activity.
• Among its identified biological processes are the tricarboxylic acid cycle and transferase activity.
• Structural studies have been performed (3D-structure keyword), and the protein has been identified through proteomics approaches.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 367–466) of human Citrate Synthase, which is conserved across human, mouse, and rat, supporting cross-reactivity.
• For Western blot (WB), a band at approximately 52 kDa is expected, though the calculated molecular weight is around 51.7 kDa.
• For IHC-P and IF/ICC, consider the mitochondrial localization; antigen retrieval methods may be necessary for paraffin-embedded sections.
• For ELISA, the antibody may be used as a capture or detection reagent; paired with an appropriate secondary antibody and optimized conditions.
• Due to the monoclonal nature, lot-to-lot consistency is expected, but assay conditions should be optimized for each experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined gateway to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-Citrate Synthase antibody for mitochondrial metabolism research. We supply optimized antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, supporting your project from concept to clinic.