Anti-CISH Rabbit Polyclonal Antibody for WB & ELISA - Q9NSE2

Core Product Specifications and Parameters

Biological Background: CISH Function and Localization

• CISH (Cytokine-inducible SH2-containing protein) is a member of the suppressor of cytokine signaling (SOCS) family, functioning as a negative feedback regulator of cytokine signal transduction.
• It specifically inhibits the JAK-STAT5 signaling pathway downstream of cytokines such as erythropoietin, prolactin, and interleukin-3 (IL-3), by suppressing STAT5 tyrosine phosphorylation.
• CISH may serve as a substrate-recognition component of an E3 ubiquitin ligase complex (SCF-like ECS), mediating ubiquitination and proteasomal degradation of target proteins.
• The protein is widely expressed in epithelial tissues, with abundant expression in liver and kidney, and lower levels in lung; distinct tissue distribution patterns exist for its isoforms.
• Key structural features include an SH2 domain and a SOCS box, which are critical for its inhibitory and ubiquitin ligase functions.
• The gene CISH undergoes alternative splicing, yielding multiple isoforms with potentially distinct roles in growth regulation and signal inhibition.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein corresponding to amino acids 1-257 of human CISH, covering the full-length protein; this suggests the antibody may recognize multiple isoforms containing this region.
• For Western blot (WB), consider that the predicted molecular weight of CISH is approximately 29 kDa, but post-translational modifications or isoform variation may affect the observed band size.
• Cross-reactivity with mouse and rat is reported, making this antibody suitable for studies in rodent models; however, validate specificity in your experimental system.
• ELISA applications may require optimization of coating and detection conditions; consider using recombinant CISH protein as a standard for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CISH polyclonal antibody supports cytokine signaling and immunology research, enabling reliable detection and assay development. Explore our comprehensive portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to accelerate your diagnostic and research workflows.