Anti-CISH Rabbit Monoclonal Antibody for WB, IP, ELISA - Q9NSE2

Core Product Specifications and Parameters

Biological Background: CISH Function and Localization

• CISH (Cytokine-inducible SH2-containing protein), also known as CIS-1, G18, or SOCS, is a member of the suppressor of cytokine signaling (SOCS) family that negatively regulates cytokine signal transduction.
• CISH specifically inhibits the JAK-STAT5 signaling pathway, suppressing tyrosine phosphorylation and transactivation of STAT5 in response to cytokines such as erythropoietin, prolactin, and interleukin-3 (IL-3).
• It functions as a substrate-recognition component of an SCF-like E3 ubiquitin ligase complex (ECS), mediating ubiquitination and proteasomal degradation of target proteins, thereby attenuating signaling.
• The protein contains an SH2 domain essential for its interaction with phosphorylated tyrosine residues on activated cytokine receptors, and a SOCS box that recruits the ubiquitin transfer machinery.
• CISH is expressed in various epithelial tissues, with abundant expression in liver and kidney, and lower levels in the lung; the distribution of alternative splice isoforms (1 and 1B) is tissue-specific.
• Keywords include alternative splicing, growth regulation, signal transduction inhibitor, and Ubl conjugation pathway, reflecting its role in modulating cell growth and protein turnover.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is 29 kDa; consider using appropriate lysis buffers and boiling conditions to ensure complete denaturation.
• When performing immunoprecipitation, cross-linking the antibody to protein A/G beads may improve capture efficiency and reduce heavy/light chain interference in subsequent detection.
• For ELISA, pair with a carefully selected detection antibody and validate the assay using recombinant CISH protein or relevant cell lysates.
• As the immunogen covers amino acids 1-170, confirm that the antibody recognizes the intended isoform and is not affected by potential N-terminal processing or modifications in your sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of high-quality monoclonal antibodies and raw materials for IVD assay development. This anti-CISH antibody supports research into cytokine signaling and immune regulation. Our sourcing capabilities include validated antibody pairs, bulk reagents, and access to rare targets to streamline your project from concept to clinic.