Anti-CISH Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NSE2

Core Product Specifications and Parameters

Biological Background: CISH Function and Localization

• CISH (Cytokine-inducible SH2-containing protein), also known as CIS-1, G18, and SOCS, is a member of the suppressor of cytokine signaling (SOCS) family.
• It functions as a negative feedback regulator of cytokine signal transduction, specifically inhibiting the JAK-STAT5 pathway activated by erythropoietin, prolactin, and interleukin 3 (IL3).
• CISH suppresses STAT5 trans-activation by blocking its tyrosine phosphorylation, thereby dampening downstream signaling.
• It may act as a substrate-recognition component of an E3 ubiquitin ligase complex, mediating ubiquitination and proteasomal degradation of target proteins.
• The protein is expressed in various epithelial tissues, with abundant expression in liver and kidney, and lower levels in lung. Isoforms 1 and 1B show distinct tissue distributions.
• Keywords associated with CISH include alternative splicing, SH2 domain, signal transduction inhibitor, and Ubl conjugation pathway, reflecting its regulatory roles.
• The protein has a molecular weight of approximately 28.7 kDa (UniProt), consistent with the observed 29 kDa.

Experimental Guidance and Technical Tips

• For Western blot (WB), a band at ~29 kDa is expected. Validate specificity using positive controls (e.g., stimulated epithelial cell lines) and negative controls (CISH knockout or siRNA-treated samples) to confirm band identity.
• In immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization conditions for the specific cell type. Include isotype controls and consider co-staining with organelle markers to visualize subcellular localization.
• For ELISA, use the antibody as a capture or detection reagent; cross-reactivity with mouse and rat CISH has been observed, but titration and validation in each species' sample matrix are recommended.
• The polyclonal nature may yield lot-to-lot variability; consider requesting a retention sample lot for consistent long-term studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-CISH antibody supports research into cytokine signaling and JAK-STAT pathways, and our team can assist with customized procurement and technical consulting for your specific assay development needs.