Anti-CISD2 Polyclonal Antibody for WB, IHC-P, ELISA - Q8N5K1

Core Product Specifications and Parameters

Biological Background: CISD2 Function and Localization

• CISD2 (CDGSH iron-sulfur domain-containing protein 2) is also known as endoplasmic reticulum intermembrane small protein (ERIS), mitoNEET-related 1 protein (Miner1), and nutrient-deprivation autophagy factor-1 (NAF-1).
• It functions as a key regulator of autophagy, antagonizing BECN1-mediated cellular autophagy at the endoplasmic reticulum.
• CISD2 participates in the interaction of BCL2 with BECN1 and is required for BCL2-mediated depression of ER Ca2+ stores during autophagy.
• It contributes to BIK-initiated autophagy but does not influence BIK-dependent caspase activation, highlighting a specific role in non-apoptotic autophagy pathways.
• CISD2 is involved in lifespan control, likely through its regulatory function in autophagy.
• The protein localizes to the endoplasmic reticulum membrane and the mitochondrial outer membrane, positioning it at critical organelle contact sites.
• CISD2 is broadly expressed in tissues including testis, small intestine, kidney, lung, brain, heart, pancreas, and platelets.
• It contains a 2Fe-2S iron-sulfur cluster and is associated with human diseases such as deafness and diabetes mellitus.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody corresponds to amino acids 61–135 of human CISD2; consider verifying sequence conservation across target species (Human, Mouse, Rat) for expected cross-reactivity.
• For Western blotting, a band at approximately 15 kDa is anticipated; optimize gel percentage (e.g., 12–15% SDS-PAGE) and transfer conditions for low molecular weight proteins.
• In immunohistochemistry (paraffin-embedded, IHC-P), antigen retrieval protocols (e.g., heat-induced epitope retrieval) may be necessary; signal distribution is expected in endoplasmic reticulum and mitochondrial compartments.
• For ELISA applications, the antibody may be suitable as a capture or detection reagent; perform checkerboard titrations with the recombinant immunogen or native target to determine optimal working concentrations.
• As a rabbit polyclonal antibody, lot-to-lot consistency should be monitored; inclusion of positive and negative controls (e.g., knockout cell lines or siRNA-treated samples) is recommended for experimental validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined sourcing channel for IVD raw materials. Beyond this anti-CISD2 antibody, we provide optimized monoclonal/polyclonal antibody pairs, bulk ancillary reagents, and support for rare autophagy and mitochondrial targets to accelerate your assay development.