Anti-CISD2 Polyclonal Antibody for WB, IHC-P, ELISA - Q8N5K1

Core Product Specifications and Parameters

Biological Background: CISD2 Function and Localization

• CISD2 (CDGSH iron-sulfur domain-containing protein 2), also known as ERIS, Miner1, and NAF-1, is a 135 amino acid protein with a predicted molecular weight of approximately 15 kDa.
• It contains a CDGSH domain that coordinates a 2Fe-2S cluster, characteristic of the mitoNEET protein family.
• CISD2 is a key regulator of autophagy, functioning at the endoplasmic reticulum (ER) by mediating the interaction between BCL2 and BECN1, which is required for BCL2-mediated depression of ER Ca²⁺ stores during autophagy.
• It participates in BIK-initiated autophagy without affecting BIK-dependent caspase activation, indicating a specific role in autophagic rather than apoptotic pathways.
• The protein localizes to both the ER membrane and the mitochondrial outer membrane, linking these organelles in cellular homeostasis.
• CISD2 is implicated in life span control and its dysfunction is associated with Wolfram syndrome 2 (WFS2), a disorder featuring diabetes mellitus and deafness.
• It is broadly expressed in tissues including testis, small intestine, kidney, lung, brain, heart, pancreas, and platelets.

Experimental Guidance and Technical Tips

• For Western blot, expect a band at approximately 15 kDa; use a positive control lysate from human, mouse, or rat cell lines or tissues known to express CISD2 (e.g., testis, kidney, or brain).
• In immunohistochemistry (IHC-P), optimize antigen retrieval (e.g., heat-induced epitope retrieval in citrate buffer pH6) and validate using tissue sections with abundant expression, such as pancreas or small intestine.
• For ELISA, consider pairing with a CISD2-specific capture antibody if designing a sandwich assay; perform a checkerboard titration to determine optimal concentrations.
• Since the immunogen covers amino acids 61–135, the antibody may recognize both endogenous and recombinantly expressed CISD2; cross-reactivity with mouse and rat homologs has been demonstrated, but additional validation is recommended for other species.
• Include appropriate positive and negative controls (e.g., CISD2 knockdown lysates or tissues) to confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, connecting you with high-quality antibodies and reagents from development to commercialization. This anti-CISD2 polyclonal antibody supports research into autophagy and mitochondrial function, areas where consistent reagent supply is critical. Beyond this product, CamelBio offers validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents to meet your assay needs.