Anti-CISD1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NZ45

Core Product Specifications and Parameters

Biological Background: CISD1 Function and Localization

• CISD1 (CDGSH iron-sulfur domain-containing protein 1), also known as mitoNEET, is a mitochondrial protein that functions as an L-cysteine transaminase, catalyzing the reversible transfer of the amino group from L-cysteine to 2-oxoglutarate to produce 2-oxo-3-sulfanylpropanoate and L-glutamate, dependent on pyridoxal 5'-phosphate (PLP) cofactor (PubMed:36194135). Related references: PMID:36194135
• Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity).
• Can transfer [2Fe-2S] clusters to apo-acceptor proteins in an oxidation state-dependent manner, likely functioning as a redox sensor that regulates mitochondrial iron-sulfur cluster assembly and iron trafficking upon oxidative stress (PubMed:17584744, PubMed:21788481, PubMed:23758282). Related references: PMID:17584744 PMID:21788481 PMID:23758282
• Localized to the mitochondrial outer membrane (signal-anchor transmembrane helix).
• Expression is reduced in cells derived from cystic fibrosis patients.
• Additional features include acetylation, isopeptide bond, and Ubl conjugation (UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to amino acids 29-108 of human CISD1. This region is located in the cytosolic domain of the protein, suggesting the antibody may recognize the native, non-transmembrane portion.
• As a polyclonal antibody, consider evaluating lot-to-lot consistency in critical applications; validate specificity in the relevant sample system (e.g., human, mouse, rat cell lysates).
• For Western blot (WB), the predicted molecular weight is ~12 kDa; ensure appropriate gel percentage and transfer conditions for small proteins.
• For immunofluorescence (IF/ICC), permeabilization is required to detect the mitochondrial outer membrane localization.
• Since CISD1 expression is reduced in cystic fibrosis-derived cells, these may serve as a negative control or disease model for expression studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined, one-stop channel for high-quality IVD raw materials, technical know-how, and project consultation—from concept to clinic. This anti-CISD1 polyclonal antibody supports research into mitochondrial iron-sulfur cluster biology and oxidative metabolism. Beyond single research-use antibodies, we offer matched antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of rare target-specific raw materials to accelerate your assay development and manufacturing workflows.