Anti-CIRH1A Rabbit Polyclonal Antibody for WB, ELISA - Q969X6

Core Product Specifications and Parameters

Biological Background: CIRH1A (UTP4) Function and Localization

• UTP4 (also known as CIRH1A, Cirhin) is a ribosome biogenesis factor that functions as part of the small subunit (SSU) processome, a large ribonucleoprotein complex essential for producing the small 40S ribosomal subunit.
• It is required for nucleolar processing of pre-18S ribosomal RNA (rRNA) at cleavage sites A', A0, 1, and 2b, and for optimal pre-rRNA transcription by RNA polymerase I. Related references: PMID:17699751, PMID:19732766, PMID:34516797
• UTP4 may also act as a transcriptional regulator, as suggested by its involvement in gene expression modulation (PubMed:22916032). Related references: PMID:22916032
• Localization: Predominantly found in the nucleolus, but it also associates with chromosomes, reflecting its dual role in ribosome biogenesis and chromatin-related functions.
• The protein contains multiple WD40 repeats, facilitating protein-protein interactions, and undergoes post-translational modifications such as phosphorylation and ubiquitination (isopeptide bond formation).
• Notably, UTP4 positively regulates HIVEP1, a transcription factor that binds to the enhancer elements of certain viral promoters, including HIV-1, SV40, and CMV, suggesting a possible role in viral replication cycles.
• Keywords: Alternative splicing, Isopeptide bond, Nucleus, Phosphoprotein, Repeat, Ribosome biogenesis, rRNA processing, Transcription, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 497‑686) of human UTP4; in Western blotting, the antibody may detect a band near the predicted 77 kDa for the full‑length protein. For optimal results, consider using nuclear or nucleolar‑enriched lysates.
• The antibody shows cross‑reactivity with mouse UTP4, making it suitable for mouse model studies. Validate species‑specific detection conditions and consider sequence homology when interpreting results.
• For ELISA applications, the recombinant immunogen can serve as a positive control. Assay conditions, including coating buffers and incubation times, should be optimized to ensure reliable performance.
• Given UTP4’s roles in ribosome biogenesis, transcriptional regulation, and potential host‑virus interactions, this antibody may be useful in studies of nucleolar biology, rRNA processing, and viral replication mechanisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies for nucleolar research and ribosome biogenesis. From CIRH1A/UTP4 polyclonal antibodies to bulk ancillary reagents and rare‑target sourcing, we provide one‑stop procurement solutions for diagnostic manufacturers and labs. Explore our catalog to find optimized monoclonal/polyclonal antibodies, antibody pairs, and technical support to accelerate your assay development—from concept to clinic.