Anti-CIRBP/CIRP Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q14011

Core Product Specifications and Parameters

Biological Background: CIRBP Function and Localization

• CIRBP (Cold-inducible RNA-binding protein), also known as CIRP or A18 hnRNP, is a glycine-rich RNA-binding protein that functions as a cold-inducible mRNA binding factor.
• It plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival (e.g., RPA2 and TXN) and acting as a translational activator. Conversely, it may act as a translational repressor. (By similarity)
• Overexpression of CIRBP promotes assembly of stress granules (SGs).
• The protein localizes to both the nucleus (nucleoplasm) and cytoplasm.
• It is ubiquitously expressed across tissues.
• Post-translational modification: Phosphoprotein.
• Keywords: Activator, Repressor, RNA-binding, Stress response, Alternative splicing.

Experimental Guidance and Technical Tips

• For western blot (WB), the predicted molecular weight is approximately 19 kDa. Consider using appropriate loading controls and validate detection in your specific sample system.
• For immunohistochemistry on paraffin-embedded sections (IHC-P), antigen retrieval conditions may need optimization; test a range of retrieval buffers and incubation times.
• For ELISA applications, ensure proper coating concentration and block/detection conditions; cross-reactivity with mouse and rat samples may be leveraged for multispecies studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated access to IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. For targets like CIRBP involved in stress response and RNA biology, we offer validated polyclonal antibodies, matched antibody pairs, recombinant proteins, and bulk ancillary reagents. Our sourcing capabilities extend to rare targets and custom development, ensuring a streamlined procurement experience. Reach out to explore how CamelBio can accelerate your assay development pipeline.