Anti-CIRBP Monoclonal Antibody for WB, IP, IF-P, IHC-P, ELISA - Q14011

Core Product Specifications and Parameters

Biological Background: CIRBP Function and Localization

• CIRBP (Cold-inducible RNA-binding protein), also known as A18 hnRNP and glycine-rich RNA-binding protein CIRP, is encoded by the CIRBP gene in humans.
• Acts as a cold-inducible mRNA binding protein, playing a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival.
• Functions as both a translational activator and repressor; it binds specifically to the 3'-UTRs of stress-responsive transcripts such as RPA2 and TXN.
• When overexpressed, promotes the assembly of stress granules (SGs), which are cytoplasmic aggregates of stalled translation preinitiation complexes.
• Localizes primarily to the nucleus and nucleoplasm, and can shuttle to the cytoplasm under stress conditions.
• Ubiquitously expressed across tissues, consistent with its role in cellular stress responses.
• Post-translational modifications include phosphorylation (as indicated by the keyword "Phosphoprotein").
• The protein has alternative splice variants, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human CIRBP protein (aa 1-172), which is ideal for detecting total CIRBP in samples.
• For Western blotting (WB), the predicted molecular weight is approximately 19 kDa; however, post-translational modifications might alter migration.
• Immunoprecipitation (IP) and ELISA applications may benefit from the high affinity and specificity of this rabbit monoclonal antibody.
• For immunofluorescence (IF) and immunohistochemistry (IHC), consider optimizing fixation and permeabilization protocols, as CIRBP can shuttle between nucleus and cytoplasm.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with seamless access to validated IVD raw materials. For stress response and RNA-binding protein studies, we offer a comprehensive portfolio including this CIRBP antibody, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Contact us to discuss your specific sourcing needs for rare targets and custom antibody development.