Anti-Cip4 Rabbit Monoclonal Antibody for WB and ELISA - Q15642

Core Product Specifications and Parameters

Biological Background: Cip4 Function and Localization

• Cip4, also known as Cdc42-interacting protein 4, is encoded by the TRIP10 gene and also referred to as STP, HSTP, STOT, and TRIP-10.
• Insulin signaling: Required for translocation of glucose transporter GLUT4 to the plasma membrane in response to insulin (by similarity).
• Endocytic membrane tubulation: Coordinates membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine to promote membrane invagination and tubule formation.
• Actin polymerization: Promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP, which activates the Arp2/3 complex; this may aid in fission of membrane tubules to form endocytic vesicles.
• Podosome formation: Required for the formation of podosomes, actin-rich adhesion structures in monocyte-derived cells, and may be involved in lysosomal retention of FASLG/FASL.
• Subcellular localization: Found in the cytoplasm, cytoskeleton, cell cortex, lysosome, Golgi apparatus, cell membrane, phagocytic cup, and perinuclear region.
• Tissue distribution: Widely expressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus, and trachea.
• Structural features: Contains an SH3 domain and a coiled coil region; is a phosphoprotein involved in lipid binding and endocytosis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of human Cip4. This antibody is expected to recognize the full-length protein and potentially N-terminal isoforms.
• Predicted molecular weight is 68 kDa; confirm by Western blot with appropriate positive controls (e.g., cell lines expressing Cip4) and use a reliable protein ladder.
• Validated applications: Western blot (WB) and ELISA. For WB, consider optimizing antibody dilution (e.g., 1:500-1:2000) and blocking conditions in your specific experimental system. For ELISA, test with recombinant protein or peptide to establish standard curves.
• Cross-reactivity is observed with human and mouse Cip4, indicating sequence conservation in the immunogen region. Validate reactivity in your target species and sample type.
• When using for endocytosis or cytoskeletal studies, consider appropriate stimulation conditions (e.g., insulin treatment for GLUT4 translocation assays).
• Store at -20°C or as recommended; avoid repeated freeze-thaw cycles to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials and technical support, from concept to clinic. This Cip4 rabbit monoclonal antibody complements our portfolio of cytoskeleton and cell biology reagents, and we can source additional validated antibody pairs, bulk ancillary reagents, or rare-target antibodies to meet your specific assay development needs. Contact us to discuss customized sourcing and supply solutions.