Anti-CINP (Cyclin-dependent kinase 2-interacting protein) Polyclonal Antibody for WB, ELISA - Q9BW66

Core Product Specifications and Parameters

Biological Background: CINP Function and Localization

• CINP is a component of the DNA replication complex that physically and functionally links CDK2 and CDC7 kinases during origin firing. Related references:
  • PMID:16082200
  • PMID:19889979
• It regulates ATR-mediated checkpoint signaling in response to DNA damage. Related references:
  • PMID:16082200
  • PMID:19889979
• As a subunit of the 55LCC heterohexameric ATPase complex, CINP promotes replisome proteostasis, which is essential for replication fork progression, sister chromatid cohesion, and chromosome stability. Related references:
  • PMID:38554706
• The ATPase activity of the 55LCC complex is specifically stimulated by replication fork DNA and is coupled to cysteine protease-dependent cleavage of replisome substrates upon replication stress. Related references:
  • PMID:38554706
• In the cytoplasm, the 55LCC complex also participates in the maturation of pre-60S ribosomal particles by facilitating the release of shuttling protein RSL24D1/RLP24. Related references:
  • PMID:35354024
• CINP localizes predominantly to the nucleus.
• The protein undergoes post-translational modifications including acetylation and phosphorylation, and alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen covers nearly the full-length human CINP protein (residues 1–212), so the polyclonal antibody is expected to recognize multiple epitopes; consider using it for both denaturing (WB) and native (ELISA) applications.
• For Western blotting, a band around 24 kDa corresponding to endogenous CINP should be detectable in human and rat lysates; validate with appropriate positive and negative controls in your sample system.
• When performing ELISA, optimize coating antigen concentration and antibody dilution; recombinant CINP protein or cell lysates can serve as target.
• Due to the polyclonal nature, lot-to-lot consistency may vary; consider including a reference standard in each experiment if quantitative comparisons are required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a complete portfolio of IVD raw materials, technical services, and consulting—from concept to clinic. For DNA replication and cell cycle studies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies (including anti-CINP), bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development and scale-up.