Anti-Cingulin Polyclonal Antibody for WB, IHC-P, ELISA - Q9P2M7

Core Product Specifications and Parameters

Biological Background: Cingulin Function and Localization

• Cingulin (CGN) is a coiled-coil protein localized on the cytoplasmic face of tight junctions (TJs) in polarized epithelia and some endothelia.
• It plays a key role in the formation and regulation of the TJ paracellular permeability barrier.
• Alternative splicing generates multiple isoforms, which may modulate junctional properties.
• Cingulin is expressed in pancreas, kidney, liver, and lung, but not in skeletal muscle, placenta, brain, or heart.
• Post-translational modifications include acetylation and phosphorylation, likely regulating its function and interactions.
• Proteomics studies have identified cingulin as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 904–1203); consider this when designing blocking or detection strategies.
• For Western blotting, full-length cingulin migrates at ~137 kDa; use appropriate gel percentage (e.g., 8–10%) and transfer conditions.
• In IHC-P, validate antigen retrieval methods and antibody dilution using TJ-positive tissues such as pancreas or kidney.
• ELISA protocols may require optimization of coating buffer and detection system; recombinant protein can be used as a positive control.
• Confirm specificity in your sample system by comparing results with genetic or siRNA controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs seamless access to IVD raw materials, technical services, and consulting—from early-stage development to clinical application. For tight junction and epithelial barrier research, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your projects.