Anti-CIDEB Rabbit Polyclonal Antibody for WB, ELISA - Q9UHD4

Core Product Specifications and Parameters

Biological Background: CIDEB Function and Localization

• CIDEB is a lipid transferase that promotes unilocular lipid droplet formation by mediating lipid droplet fusion, enhancing lipid storage and restricting lipolysis. Related references: PMID:35939579
• It localizes to lipid droplet contact sites where it facilitates directional neutral lipid transfer from smaller to larger droplets, driven by internal pressure differences.
• CIDEB is required for very-low-density lipoprotein (VLDL) lipidation and maturation, and is involved in the biogenesis of VLDL transport vesicles.
• The protein resides on lipid droplets, endoplasmic reticulum membranes, Golgi apparatus, and COPI-coated vesicles, indicating a role in cytoplasmic vesicle transport.
• It may activate apoptosis, as indicated by its alternative name Cell death-inducing DFFA-like effector B. Related references: PMID:10619428
• CIDEB is highly expressed in liver and small intestine, with lower expression in colon, kidney, and spleen.
• It is involved in Hepatitis C virus (HCV) entry into hepatocytes and viral assembly.
• Keywords include phosphoprotein, suggesting post-translational regulation by phosphorylation.

Experimental Guidance and Technical Tips

• For Western blot (WB), a band of approximately 25 kDa is expected; consider using hepatocyte cell lines (e.g., HepG2) or liver tissue lysates as positive controls.
• For ELISA, the recombinant immunogen (amino acids 80-160) may be used as a standard for antibody titration and specificity assessment.
• Cross-reactivity is reported for human; for other species, validation in the relevant sample system is recommended.
• Store at recommended conditions and avoid repeated freeze-thaw cycles to maintain antibody activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material sourcing for diagnostic manufacturers and research laboratories, covering every stage from concept to clinic. This anti-CIDEB polyclonal antibody is part of our portfolio for metabolism, liver disease, and infectious disease research. We offer validated antibody pairs, bulk ancillary reagents, and customized procurement for rare targets, ensuring a reliable supply chain to accelerate your assay development.