Anti-CIDEB Polyclonal Antibody for WB, ELISA - Q9UHD4

Core Product Specifications and Parameters

Biological Background: CIDEB Function and Localization

• CIDEB is a lipid transferase that promotes unilocular lipid droplet formation by mediating lipid droplet fusion, a key process in hepatocyte lipid storage. Related references: PMID:35939579
• It localizes to lipid droplets, endoplasmic reticulum membranes, the Golgi apparatus, and COPI-coated vesicles, facilitating intracellular lipid trafficking.
• CIDEB is highly expressed in liver and small intestine, with lower levels in colon, kidney, and spleen, reflecting its tissue-specific metabolic roles.
• Also known as cell death activator CIDE-B, it may activate apoptosis, highlighting a dual functional role. Related references: PMID:10619428
• Required for very-low-density lipoprotein (VLDL) lipidation and maturation, aiding in hepatic lipid export.
• Phosphorylation and other post-translational modifications regulate its activity; it also plays a role in host-virus interactions, such as Hepatitis C virus entry.
• Belongs to the CIDE family of proteins, which share roles in lipid metabolism and cell death pathways.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 80–160; expect detection of full-length CIDEB at approximately 25 kDa in western blotting, with potential for cleaved or modified forms.
• For WB, consider using reducing conditions and liver lysates as a positive control due to high hepatic expression.
• Cross-reactivity with mouse enables validation in murine models; however, confirm specificity under your experimental conditions.
• ELISA applications may utilize this antibody as a capture or detection reagent; validate with recombinant CIDEB protein to ensure sensitivity and specificity.
• Follow standard antibody handling: store at recommended temperatures, minimize freeze-thaw cycles, and spin briefly before use to remove particulates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing for the research and diagnostic communities. For investigations into hepatocyte biology and lipid metabolism, we offer specialized reagents like this CIDEB antibody to support studies on lipid droplet dynamics and VLDL assembly. Our one-stop sourcing model streamlines procurement from concept to clinic, ensuring consistent quality and reliable supply for your assay development needs.