Anti-CIDEA Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O60543

Core Product Specifications and Parameters

Biological Background: CIDEA Function and Localization

• CIDEA (Cell death activator CIDE-A) is a lipid transferase that promotes unilocular lipid droplet formation by mediating lipid droplet fusion, thereby restricting lipolysis and favoring lipid storage. Related references: PMID:19843876 PMID:26118629
• It localizes to the surface of lipid droplets and at contact sites between adjacent droplets, facilitating directional net neutral lipid transfer from smaller to larger droplets.
• CIDEA may also function as a CEBPB coactivator in epithelial cells, regulating expression of target genes such as ID2, IGF1, PRLR, SOCS1, SOCS3, and XDH, but not casein.
• The protein is found in both the lipid droplet and the nucleus, suggesting dual roles in lipid metabolism and transcriptional regulation.
• Overexpression of CIDEA induces apoptosis, although the physiological significance of this apoptotic function remains unclear.
• CIDEA is expressed in omental and subcutaneous adipose tissue at the protein level, consistent with its role in lipid storage.
• Key structural features include a CIDE-N domain involved in lipid droplet fusion and a CIDE-C domain mediating protein–protein interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20-219 of human CIDEA, covering the majority of the protein, which may enable recognition of potential isoforms and cleaved fragments.
• For Western blotting, a band at approximately 25 kDa is expected; whole cell lysates from human, mouse, or rat adipose tissue are suggested as positive controls.
• In IHC-P, consider antigen retrieval methods and validate in paraffin-embedded adipose tissue sections; background may be reduced by optimizing blocking conditions.
• For ELISA, the antibody can be employed as a capture or detection reagent; pairing with a matched antibody pair may enhance sensitivity—validate using recombinant CIDEA protein.
• The antibody cross-reacts with mouse and rat, but users should confirm reactivity in their specific sample type with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop provider of IVD raw materials, offering comprehensive solutions from concept to clinic. This anti-CIDEA antibody supports research in lipid metabolism, obesity, and apoptosis, and we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to accelerate your diagnostic development. Our global sourcing network also ensures access to rare target reagents, streamlining your procurement process.