Anti-CIC (Capicua) Rabbit Polyclonal Antibody for WB and ELISA - Q96RK0

Core Product Specifications and Parameters

Biological Background: CIC (Capicua) Function and Localization

• Protein capicua homolog (CIC), also known as MRD45, is a transcriptional repressor encoded by the CIC gene (KIAA0306) in humans.
• CIC functions as a DNA-binding transcriptional repressor that plays a critical role in central nervous system (CNS) development, acting in concert with ATXN1 and ATXN1L to regulate brain development.
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• CIC is expressed in fetal brain, highlighting its developmental importance in neural tissues.
• Key post-translational modifications include acetylation, methylation, and phosphorylation, which may modulate its repressor activity and protein interactions.
• The protein is associated with several molecular functions and pathologies, including DNA binding, alternative splicing, and disease variants linked to intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1379–1608 within the C-terminal region of human CIC (NP_055940.3). Consider this when designing detection strategies for full-length versus truncated isoforms.
• For Western blot, use whole-cell or nuclear extracts from brain or neuronal cell lines. Validate antibody specificity using lysates from CIC-expressing and knockdown/knockout systems.
• In ELISA, the antibody may serve as a capture or detection reagent. Optimize coating conditions and check cross-reactivity in mouse samples if using mouse-derived matrices.
• Note that the supplied protein weight (164 kDa) may differ from the canonical UniProt molecular weight (258 kDa). Verify band size in your experimental system, as post-translational modifications or alternative splicing can shift migration.

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CamelBio offers comprehensive access to IVD raw materials, supporting diagnostic manufacturers and research laboratories with a reliable supply of validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. Whether you are developing assays targeting transcriptional regulators like CIC for neuroscience applications or require rare-target raw material sourcing, our platform streamlines procurement from concept to clinic. Explore our catalog for CNS development-related antibodies and let us assist with your end-to-end sourcing needs.