Anti-CIAPIN1 Polyclonal Antibody for WB, IF/ICC - Q6FI81

Core Product Specifications and Parameters

Biological Background: Anamorsin (CIAPIN1) Function and Localization

• Anamorsin (CIAPIN1), also known as DRE2 homolog, is a component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery essential for maturation of extramitochondrial Fe-S proteins. It facilitates de novo assembly of [4Fe-4S] clusters on the scaffold complex NUBP1-NUBP2.
  Related references:
  PMID:23596212
• CIAPIN1 functions as part of an electron transfer chain, receiving electrons from NADPH via the flavoprotein NDOR1, which are then used for Fe-S cluster biogenesis. This system is also required for assembly of the diferric tyrosyl radical cofactor of ribonucleotide reductase (RNR).
• The protein exhibits anti-apoptotic activity, negatively regulating cell death upon cytokine withdrawal, and is involved in promoting hematopoietic cell development.
• CIAPIN1 is widely expressed, with high levels in heart, liver, and pancreas, and localizes to the cytoplasm, nucleus, and mitochondrial intermembrane space.
• The gene undergoes alternative splicing, producing multiple isoforms, and the protein is subject to phosphorylation.
• Anamorsin binds iron-sulfur clusters, including [2Fe-2S] and [4Fe-4S] cofactors, marking it as a key metal-binding protein in cellular iron homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human CIAPIN1 protein (residues 1–312), suggesting broad epitope coverage. Due to the polyclonal nature, multiple epitopes may be recognized, which can enhance detection sensitivity but also potential cross-reactivity; confirm specificity in your model system.
• For Western blotting, a band around 34 kDa is anticipated based on the calculated molecular weight. However, post-translational modifications or isoform variations may shift the observed band; consider using positive control lysates from human cell lines.
• In immunofluorescence/ICC, CIAPIN1 is reported in the cytoplasm, nucleus, and mitochondria; validate subcellular distribution under your experimental conditions. Co-staining with organelle markers is recommended.
• For immunoprecipitation, the antibody may effectively pull down CIAPIN1 and potentially interacting proteins; include proper controls to exclude non-specific binding.
• When using ELISA, the antibody's sensitivity and linearity should be validated with recombinant CIAPIN1 or known positive samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic and research institutions to provide validated IVD raw materials, including optimized polyclonal antibodies like this CIAPIN1 rabbit pAb, ideal for investigating iron-sulfur cluster assembly and apoptosis pathways. Beyond standard catalog products, we source rare-target reagents, bulk ancillary materials, and offer technical consulting from concept to clinical application. For your Fe-S biogenesis or cell death studies, rely on CamelBio as your one-stop supply chain bridge.