Anti-cIAP2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13489

Core Product Specifications and Parameters

Biological Background: cIAP2 Function and Localization

• Baculoviral IAP repeat-containing protein 3 (BIRC3), also known as cIAP2, API2, and hIAP1, is a member of the inhibitor of apoptosis (IAP) family characterized by the presence of one or more baculovirus IAP repeat (BIR) domains and a RING finger domain.
• Functions as a multi-functional regulator of apoptosis, inflammatory signaling, and immunity by acting as an E3 ubiquitin-protein ligase, mediating ubiquitination of key signaling molecules such as RIPK1, RIPK2, CASP3, CASP7, CASP8, IKBKE, TRAF1, and BCL10.
• Positively regulates canonical NF-kB signaling while suppressing constitutive activation of the non-canonical pathway, thereby balancing inflammatory and cell survival responses.
• Plays a critical role in innate immunity by regulating pattern recognition receptors (PRRs), including Toll-like receptors (TLRs), NOD-like receptors (NLRs), and RIG-I-like receptors (RLRs), influencing downstream cytokine production.
• Protects cells from spontaneous assembly of the ripoptosome, a death-inducing complex, by ubiquitinating RIPK1 and CASP8.
• Involved in modulating cell proliferation, invasion, and metastasis, linking its activity to cancer biology.
• Localizes to both the cytoplasm and the nucleus, consistent with its diverse signaling and regulatory roles.
• Highly expressed in fetal lung and kidney, and in adult lymphoid tissues including spleen, thymus, and peripheral blood lymphocytes, reflecting its importance in immune system development and function.

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 100–130 of human cIAP2 (NP_001156.1), a region within the BIR domains; the resulting polyclonal antibody is expected to recognize the full-length protein (~68 kDa) in Western blotting.
• For Western blot (WB), use 5% nonfat milk or BSA for blocking and overnight primary incubation at 4°C; a band at approximately 68 kDa should be detected. Consider using lysates from cells with known cIAP2 expression (e.g., lymphoid lines) as positive controls.
• For immunohistochemistry (IHC-P), antigen retrieval may be required; validate staining specificity with tissue sections from spleen or thymus and compare with normal IgG controls.
• In immunofluorescence (IF/ICC), observe cytoplasmic and nuclear localization; co-staining with organelle markers can confirm subcellular distribution.
• For ELISA, the antibody may be used as a capture or detection reagent; optimize coating concentrations and blocking conditions for your specific assay format.
• Cross-reactivity with mouse and rat is anticipated; however, performance in these species should be verified experimentally under your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering everything from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents and technical consulting. Designed for apoptosis and NF-κB research, this anti-cIAP2 polyclonal antibody exemplifies our commitment to providing high-quality reagents for diagnostic and life science applications. Whether you need routine antibodies or rare-target raw materials, CamelBio bridges the gap between concept and clinic.