Anti-CIAO1 Rabbit Polyclonal Antibody for WB, ELISA - O76071

Core Product Specifications and Parameters

Biological Background: CIAO1 (CIA1/WDR39) Function and Localization

• CIAO1 (gene: CIAO1; also known as CIA1, WDR39) encodes a WD repeat-containing protein that is a probable cytosolic iron-sulfur protein assembly factor, key for extramitochondrial Fe/S cluster incorporation.
  Related references: PMID:17937914 PMID:23891004 PMID:38950322

• Depending on its binding partners, CIAO1 directs Fe/S cluster insertion into different client proteins: the CIAO1-CIAO2B-MMS19 complex targets the majority of cytosolic-nuclear Fe/S proteins, whereas the CIAO1-CIAO2A branch specifically matures ACO1 and stabilizes IREB2.
  Related references: PMID:23891004

• CIAO1 has been reported to interact with the Wilms tumor transcription factor WT1, modulating its transactivation activity.
  Related references: PMID:9556563

• As part of the MMXD complex, CIAO1 may play a role in mitotic chromosome segregation.
  Related references: PMID:20797633

• Immunofluorescence and biochemical fractionation studies confirm a cytoplasmic localization for CIAO1.

• The protein is characterized by WD repeat domains and has a computed molecular mass of ~38 kDa (UniProt: O76071).

• Related UniProt keywords include 3D-structure, chromosome partition, and disease variant, reflecting its structural, mitotic, and clinical relevance.

Experimental Guidance and Technical Tips

• The polyclonal antibody, raised against a recombinant protein covering amino acids 1–339 of human CIAO1, is expected to detect endogenous CIAO1 at ~38 kDa in western blotting (WB) and perform in ELISA assays.
• Given its cross-reactivity with mouse and rat, verify performance in your species of interest using appropriate positive controls (e.g., HeLa, HEK293, or tissue lysates).
• As a cytoplasmic protein, whole-cell lysates prepared under standard reducing or non-reducing conditions should be suitable; optimize blocking, antibody dilution (e.g., 1:500–1:2000), and detection conditions empirically.
• For ELISA, consider coating with recombinant CIAO1 or using a capture-detection pair setup; validate specificity with competitor proteins or knockout samples when possible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, providing validated antibodies like this anti-CIAO1 polyclonal for research and diagnostic development. Beyond single reagents, we offer antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing hard-to-find raw materials for iron-sulfur cluster biology and chromosome segregation research. Let CamelBio bridge your project from concept to clinic with reliable supply solutions.