Anti-CHX10 (VSX2) Rabbit Monoclonal Antibody for WB and ELISA - P58304

Core Product Specifications and Parameters

Biological Background: Visual System Homeobox 2 Function and Localization

• VSX2 (also known as CHX10, HOX10, RET1) is a homeobox transcription factor that binds the DNA consensus sequence 5'-[TC]TAATT[AG][AG]-3' to regulate gene expression. Related references: PMID:27301076
• It plays a significant role in the specification and morphogenesis of the sensory retina.
• VSX2 contributes to the maintenance of neural retina identity by regulating canonical Wnt genes and CTNNB1 localization. Related references: PMID:27301076
• In spinal cord development, VSX2 mediates differentiation of V2a interneurons by repressing motor neuron gene transcription through competitive binding to ISL1-LHX3 response elements. Related references: PMID:17919464, PMID:27477290
• It represses SAG transcription by binding the photoreceptor conserved element-1 (PCE-1) and competitively inhibiting ISL1-LHX3 complexes. Related references: PMID:16236706, PMID:27477290
• VSX2 acts as a positive transcriptional regulator of NXNL1 (in synergy with VSX1) and a negative regulator of MITF.
• The protein localizes to the nucleus and is abundantly expressed in retinal neuroblasts during development and in the inner nuclear layer of the adult retina, particularly in bipolar cells.
• Associated keywords include: DNA-binding, homeobox, developmental protein, activator, repressor, microphthalmia, cataract.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 262–361 of human CHX10; confirm that the antibody recognizes the full-length protein in your sample system, as the epitope may be masked or absent in certain isoforms.
• For Western blotting, a band near 39 kDa is expected; run appropriate molecular weight markers and verify specificity with knockdown or knockout controls if available.
• Cross-reactivity with mouse and rat is reported; if using these species, validate performance under your experimental conditions.
• For ELISA applications, optimize coating antigen concentration and antibody dilution to achieve desired sensitivity and linear range.
• As a rabbit monoclonal antibody, it offers high affinity and reproducibility; consider paired detection with a suitable secondary antibody or direct conjugation for multiplex assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, from concept to clinic. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for vision and developmental biology research. If you need a rare target raw material like CHX10 (VSX2) or custom reagent development, contact our team for tailored solutions.