Anti-CHUK (IKKα) Polyclonal Antibody for IF/ICC, IF-P, ELISA - G3V926

Core Product Specifications and Parameters

Biological Background: CHUK/IKKα Function and Localization

• CHUK (conserved helix-loop-helix ubiquitous kinase), also known as IKKα, IκBKA, or I-kappa-B kinase 1, is encoded by the Chuk gene. It is the inhibitor of nuclear factor kappa-B kinase subunit alpha.
• The protein is a serine/threonine protein kinase with ATP-binding and nucleotide-binding domains, classified as a transferase.
• Subcellular localization includes both the cytoplasm and nucleus, indicating dynamic shuttling potential.
• It is a phosphoprotein, suggesting regulation by phosphorylation events.
• The rat protein has a predicted molecular weight of approximately 84.8 kDa (UniProt), but the target band for this antibody is observed at ~78 kDa.
• As indicated by its full name, CHUK functions as the inhibitor of nuclear factor kappa-B kinase subunit alpha, implicating it in the NF-κB signaling cascade.
• Alternative names include I-kappa-B kinase 1 and Nuclear factor NF-kappa-B inhibitor kinase alpha.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a recombinant fragment corresponding to amino acids 400-660 of rat CHUK, covering part of the kinase domain and possibly regulatory regions. This region shows high sequence conservation across human and mouse, consistent with the observed cross-reactivity.
• Application notes: The antibody is qualified for immunofluorescence (IF/ICC and IF-P) and ELISA. For IF, optimize fixation and permeabilization protocols to detect the target's nuclear and cytoplasmic distribution. For ELISA, it can be used for detection of recombinant or endogenous CHUK; consider pairing with an appropriate secondary antibody.
• As a polyclonal antibody, lot-to-lot consistency should be monitored, and titration is recommended for each new application or sample type.
• No validation for Western blotting is provided; if attempting WB, test with both denatured and native conditions, as the immunogen is a recombinant protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to IVD raw materials, technical services, and consulting. This anti-CHUK antibody supports NF-κB signaling research and assay development. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your workflow from concept to clinic.