Anti-CHTF18 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8WVB6

Core Product Specifications and Parameters

Biological Background: CHTF18 Function and Localization

• CHTF18 (Chromosome transmission fidelity protein 18 homolog), also known as CHL12, is a key factor in sister chromatid cohesion and ensures accurate chromosome segregation during cell division.
• It is an integral component of the CTF18-RFC complex (together with CTF8, DCC1, RFC2-5), an alternative PCNA loader that binds primed and single-stranded DNA and exhibits DNA-dependent ATPase activity stimulated by RPA and PCNA.
• The CTF18-RFC complex catalyzes ATP-dependent loading of PCNA onto gapped and primed DNA, playing a critical role in DNA replication and repair processes.
• CHTF18 directly interacts with and stimulates DNA polymerase POLH, and is involved in recruiting DNA polymerase POLE to sites of local DNA damage during repair synthesis. Related references: PMID:20227374
• CHTF18 localizes predominantly to the nucleus, consistent with its function in genome maintenance.
• It is a phosphoprotein containing ATP-binding and DNA-binding domains, and undergoes alternative splicing to produce multiple isoforms, as indicated by proteomics identification and 3D-structure studies (UniProt Keywords: 3D-structure, Alternative splicing, ATP-binding, DNA-binding, Nucleotide-binding, Nucleus, Phosphoprotein, Reference proteome).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 794-975 in the C-terminal region of human CHTF18; verify antibody specificity in samples expressing full-length and truncated isoforms.
• For Western blotting, the predicted molecular weight is ~107 kDa; use an appropriate polyacrylamide gel percentage (e.g., 8-10%) and optimize transfer conditions for high-molecular-weight proteins.
• In immunofluorescence/ICC, CHTF18 shows nuclear staining; choose fixation and permeabilization protocols that preserve nuclear antigens, and validate in the relevant cell line (e.g., HeLa or other human/rat cells).
• For ELISA, confirm binding to recombinant CHTF18 or target antigen, and titrate antibody concentration to establish optimal sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD manufacturers and research institutions with a full spectrum of raw materials, technical services, and consulting for diagnostic development. This anti-CHTF18 rabbit polyclonal antibody is one example from our extensive portfolio for DNA replication, chromatin dynamics, and cell cycle research. We also supply validated antibody pairs, bulk monoclonal and polyclonal antibodies, and specialized ancillary reagents to streamline your assay pipeline from early-stage discovery to clinical application.